Optimizing impermeant support in an intraluminal preservation solution tailored to the small intestine

  • Author / Creator
    Kokotilo, Matthew
  • The AA (amino acid) solution is an experimental preservation solution delivered intraluminally and tailored to the metabolic requirements of the small bowel. Preservation solutions contain impermeant molecules to prevent edema formation. Dextrans and hydroxyethylstarches are two such oncotic agents. The effectiveness of these molecules has never been compared, nor has the appropriate concentration ever been established within the AA solution. Rat intestines were harvested after an intravascular flush with University of Wisconsin (UW) solution and flushed intraluminally with UW or AA solution containing either dextran70 (D70; MW= 70 kDa) or hydroxyethylstarch (HES; MW= 2,200 kDa). Fluorescent microscopy, energetics, oxidative stress, and histology revealed the superiority of the HES-supplemented AA solution. In a subsequent experiment, tissues were preserved with AA solution containing 0, 2.5, 5, or 10% HES. Energy parameters and histology were superior in the 5% group. Together, these studies support the use of 5% HES within the AA solution.

  • Subjects / Keywords
  • Graduation date
    Fall 2012
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Specialization
    • Experimental Surgery
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Dr. Thomas Mueller (Department of Medicine)
    • Dr. Rachel G. Khadaroo (Department of Surgery)