Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling

  • Author / Creator
    Zheng, Jiamin
  • The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been further developed and applied for qualitative and quantitative profiling of amine- and phenol-containing metabolites by liquid chromatography electrospray ionization Fourier Transform ion cyclotron resonance MS (LC-ESI FT-ICR-MS). For labeled metabolites, a new technique using skimmer-region fragmentation, followed by tandem MS analysis of formed fragment ions, was developed for generating structural information. In addition, a new method based on use of this isotope labeling LC-MS platform was developed for human salivary metabolome analysis. It offered superior performance over other reported methods for saliva metabolome profiling. Its potential utility for disease biomarker discovery was demonstrated in the analysis of metabolomic differences between normal individuals and diseased-individuals with mild cognitive impairment (MCI).

  • Subjects / Keywords
  • Graduation date
    Fall 2012
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Dixon, Roger (Psychology)
    • Harrison, Jed (Chemistry)