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Modular Design and Directed Evolution for the Development of Genetically Encodable Fluorescent Sensors

  • Author / Creator
    Zarowny,Landon C A
  • The development of genetically encodable sensors for optical imaging has revolutionized the way researchers investigate cellular phenomena. Illuminating the molecular dynamics of the cell has become nearly routine with countless improvements in sensor design and directed evolution efforts to enhance their reporting ability. These optical technologies owe their impressive versatility to their genetically encodable nature. As new luminescent and fluorescent reporter domains are discovered, new sensors will inevitably be developed from them. In this thesis, I cover the basics of sensor design while commenting on methods for construction of prototypes, rational structure guided design, directed evolution methods, and briefly touch on new methods for developing split sensors. The goal of this thesis is to explore the currently available technologies for developing genetically encodable optical sensors and provide a methodology for general sensor design with examples provided within each chapter.

  • Subjects / Keywords
  • Graduation date
    Spring 2019
  • Type of Item
    Thesis
  • Degree
    Doctor of Philosophy
  • DOI
    https://doi.org/10.7939/r3-0myw-9e87
  • License
    Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.